Abstract
Background: Carbon dioxide (CO2) plays an important role in the host-seeking process of opportunistic, zoophilic
and anthropophilic mosquito species and is, therefore, commonly added to mosquito sampling tools. The African
malaria vector Anopheles gambiae sensu stricto is attracted to human volatiles augmented by CO2. This study
investigated whether CO2, usually supplied from gas cylinders acquired from commercial industry, could be
replaced by CO2 derived from fermenting yeast (yeast-produced CO2).