Rooting of tissue culture propagules derived from adventitious shoot cultures or callus organogenesis is an essential step for the establishment of independently ex vitro grown plants. Usually a greenhouse environment with high moisture and low light intensity is preferred for the growth of in vitro pre-rooted plants (with root primordia or initially grown roots). Therfore, a programmed mist-spray system plus shedding materials constructed in the greenhouse is needed and a root induction treatment for the propagules is also required. As search through the references shows that Camloh et al. (1994), Jambor Benczur et al. (1995) and Huang (2004) all used an auxin stimulated rooting treatment to achieve better rooting performance in P. bifurcatum, but this step is not necessary for some other Platycerium species.