For the boiling fixation, fresh leaves (1 g) were put in a plastic tube with lid, 1.5 mL boiling loading solvent was added into the tube, and the tube was kept in a boiling water bath for 5 min
For the boiling fixation, fresh leaves (1 g) were put in aplastic tube with lid, 1.5 mL boiling loading solvent was added intothe tube, and the tube was kept in a boiling water bath for 5 min