Breeding lines with high anthocyanin accumulation exhibit low yield, conditioned by the negative influence of genes linked to genes for grain color on the chromosome or its segment from the wild species. The colored grain trait has not been observed in Indian wheat cultivars. Exotic wheat lines have a very low yield and adaptation to the Indian environment. The main objective of our study was to develop and characterize Indian wheat breeding lines with satisfactory yield level and high anthocyanin content. In this manuscript we have generated several Indian colored wheat lines with good yield potential and further characterized these lines morphologically, biochemically, and microscopically.