Evaluation of antimelanogenesis activity in B16F10
GAE exhibited antimelanogenesis in human epidermal
melanocytes. B16F10 melanoma cells are widely-used to
evaluate antimelanogenic effect of test materials because
they are relatively easy to culture in vitro, and they share
most of the melanogenic mechanisms of normal human
melanocytes. To demonstrate the depigmenting activity
of GAE in B16 melanoma cells, cell viability, melanin
content, and tyrosinase activity were determined.