• Could you please explain the purification workflow in your lab ?
• How many samples per day do you want to purify on one system?
• How many milligrams of crude mix you would like purify in one injection
• What are your preferred column dimensions ?
• What is your planned injection volume ?
• What are your preferred flow rates ?
• From where do you get your analytical information ?
• In which data format do you obtain analytical data of samples ?
• How do you plan your scale up process ?
• How do you determine the purity of the collected fractions ?
• How do you handle your collected fractions (pooling, dry down)?
• Will the operators be experts in chromatography or walk up users ?
• How to purify more samples if you are the only expert chromatographer ?
• What are your plans to minimize human errors during the purification process ?
• How would you like to increase the efficiency of the purification process?