In order to ascertain if HT was the most effective protease preparation for the generation of small peptides that may potentially yield bioactive properties, meat myofibrillar and connective tissue extracts were incubated with each of the four protease preparations previously studied (AFP,FPII, F60K and HT) as well as an additional Aspergillus oryzae derived fungal protease preparation (F31K) for 24 h.