We have demonstrated that the conducting PPy having amine functional groups can be utilized as a suitable matrix for the covalent entrapment of enzyme, urease, through peptide linkage. This efficient covalent bonding of the enzyme with amine functionalized porous PTS-doped PPy film; lead the enzyme electrode to exhibit a good performance in terms of dynamic range of detection, short response time (40 s) and long lifetime stability. The low cost and simple method of fabrication of immobilizing material is an additional advantage with respect to conventional electrodes. The experiments are presently in progress to further improve the shelf lifetime of this Urs/PAPCP/ITO electrode beyond 2 months at 4–5 °C.