2.3.2. Gas chromatography–mass spectrometry (GC–MS) identification
of volatiles compounds
GC–MS analysis was carried out using an Agilent GC 7890A series
combined with an Agilent 5975C Mass Spectrometer in EI (electron
Impact) mode. The temperature of ion source and injector was 230
and 250 °C, respectively. The capillary column used was a DB-5 ms
(60 m×0.320 mm i.d and 1 μm, film thickness, J&W Scientific, Agilent
Technologies, USA). The oven temperature was programmed at 40 °C
for 5 min, then to 115 °C at 10 °C/min for 0 min continuing with
increase to 200 °C at 5 °C/min and finally to 280 °C at 10 °C/min at
which it remained for 3 min. Helium was used as the carrier gas at a
flow rate of 0.8 mL/min. The injector was operated in the split mode