Although
ionized radiation has a direct ionizing effect while neutral radiation
has indirect ionizing effect on the target [15], even such as
fluorescent proteins, a color change from green to ‘‘white’’ of pGFP
under non-ionizing UV simply indicates DNA damage of the protein,
regardless of details, to cause the green fluorescent protein
to be not excited at the green wavelength. To check the DNA transferring
efficiency, the same amount (70 ll) of DNA-transferred bacteria
for each condition was first diluted, then smeared on 16
plates, and counted for the numbers of green and ‘‘white’’ colonies
on total 16 plates.
Althoughionized radiation has a direct ionizing effect while neutral radiationhas indirect ionizing effect on the target [15], even such asfluorescent proteins, a color change from green to ‘‘white’’ of pGFPunder non-ionizing UV simply indicates DNA damage of the protein,regardless of details, to cause the green fluorescent proteinto be not excited at the green wavelength. To check the DNA transferringefficiency, the same amount (70 ll) of DNA-transferred bacteriafor each condition was first diluted, then smeared on 16plates, and counted for the numbers of green and ‘‘white’’ colonieson total 16 plates.
การแปล กรุณารอสักครู่..
