In this study, lipase from C. rugosa was immobilized on vari- ous cellulose/lignin hydrogel beads prepared by using [Emim][Ac]. The loaded content, activity, and stability of immobilized lipase were greatly enhanced by increasing the lignin content in the gel- forming solution containing cellulose and lignin. The hydrophobic nature of lignin may induce the interfacial activation of lipase and increase the interaction between lipase and hydrogel beads. In particular, the stability of lipase immobilized on cellulose/lignin hydrogel beads was significantly enhanced at low pH value.