Lactic
acid bacteria were determined by sample incorporation (ISO 13408
E) on Man, Rogosa and Sharpe agar (MRS) (Biokar), incubated at
30 C during 3 days. Catalase test was done on presumptive lactic
acid bacteria. Yeasts and moulds were enumerated on Chloramphenicol
Rose Bengal Agar Base (CRB) (Oxoid), supplemented with
chloramphenicol (100 mg l1) and incubated during 5 days at 25 C
(Portuguese Norm 3277-1)