This mixture was given an initial stir to ensure that all kernels were wetted with hexane. Conical
flask openings were covered with aluminium foil and placed on the orbital mixture shaker machine for
shaking and the samples were left to run at least 20 to 24 hours. Then the hexane/oil mixtures were
collected, filtered and decant into aluminium foil containers for solvent evaporation and placed under the
fume hood as shown in Fig. 4 for 20-24 hours. Ashwath [17] and Jahirul et al. [18] conducted the
chemical oil extraction technique using n-hexane to extract oil from beauty leaf seeds (Calophyllum
inophyllum). Hexane was again added to the conical flask of kernels, but at a ratio of 1:1 for the second
extraction by applying the same procedure. In this way, up to fifth extraction was completed by following
the similar procedure for recovery of the oil. When it was determined that the hexane had been fully
evaporated the oil was transferred into containers for further analysis.