These primers
were expected to give products of 311 bp. PCR amplifications were
performed with an initial denaturation at 95 ◦C for 4min, followed
by 35 cycles at 94 ◦C for 30 s, 55 ◦C for 30 s, 72 ◦C for 30 s and final
extension at 72 ◦C for 10 min. PCR products were separated by electrophoresis
on a 1% (w/v) agarose gel.