Agar dilution method. The agar dilution method consisted
of adding different amounts of pure essential oils dissolved in absolute
ethyl alcohol, 5% Tween 80 (v/v), to potato dextrose agar
(PDA) before pouring it into Petri dishes (8 cm diameter) while the
medium was still warm (40e50 C), to obtain concentrations of
100, 200, 400, and 800 mL L1. A 5 mm diameter disk of inoculums
of the test fungus with a cork borer, cut from the periphery of an
actively growing culture on PDA plates, was placed at the center in
each Petri plate. Control group consists of just PDA. Then the colony
radius was measured in 1e6 days at 25 C. The percentage of inhibition
of mycelia growth was calculated from mean values of
colony diameter in treated and control Petri dishes.