after growing of A. bisporus on wheat straw on mushroom farms interconnection of tubules disappearedethe strong adhesion connection between the tubules was disrupted (Fig. 2 centre) resulting in a porous structure. The structure of wheat straw becomes even more disordered after cultivation of Trichoderma spp. and A. niger on SMC, as shown
in Fig. 2. Major changes in wheat straw structure were visualized after growth of T. harzianum T10 and Trichoderma sp. group 3 TH2 isolates and minor changes were observed with isolate Trichoderma sp. group 3 T55. The tubules of wheat straw lost their parallel structure after growth of Trichoderma sp. group 3 TH2.
3.3.2. Parameters of fermentation efficiency
Polysaccharides in SMC (cellulose and hemicellulose) were hydrolysed by enzymes produced during growth of isolates of Trichoderma spp. and A. niger, whichwas followed by determination of the concentration of released reducing sugars. There were differences in the release of sugars between the examined fungal species (Fig. 3A). T. konigii TK1 released the highest level of reducing carbohydrates during SSF on SMC. Levels of protein in crude extracts at the end of SSF are given in Fig. 3B. All Trichoderma spp. isolates produced higher levels of protein than A. niger. Isolates of T. atroviride T42 and T. konigii TK1 produced the highest levels of protein rendering them as potentially good enzyme producers.