As shown in Table 2, sperm viability at 30 minutes after
thawing was significantly (P < 0.05) increased by AA when
both freezing and thawing media were supplemented, but
not when AA was only added to freezing media (i.e., AA).
At 30 minutes after thawing, GSH supplementation in
freezing and, to a greater extent, in both freezing and
thawing media, significantly (P < 0.05) increased the percentage
of viable spermatozoa when compared with the
control group.
At 240 minutes after thawing, significant (P < 0.05)
differences among control, AA, and GSH treatments were
observed, with a progressive increase in sperm viability
from control to AA and GSH groups. In addition, a significant
(P < 0.05) decrease in sperm viability from 30 to
240 minutes was observed in all treatments, except when
both freezing and thawing media were supplemented with
GSH and AA.