This study is the first to describe the optimum temperature for the growth and condition of TGGG hybrid grouper. The LWR data showed that the pellet and shrimp fed TGGG hybrid grouper exhibited relatively better growth form and condition at 26°C and 30°C than at 22°C and 34°C. The best growth and condition were observed among shrimp-fed fish at 30°C. Therefore, TGGG hybrid grouper is suggested to culture at 30°C feeding shrimp diet although the cost of the diet needed to be considered. These findings may help grouper farmers and hatchery owners improve the growth of TGGG hybrid grouper under aquaculture conditions not only in Malaysia, but also in other nearby countries.