3. Result and discussion
3.1. Lacto-propionic orcein staining
First, for the experiments of chromosome observation at the metaphase stage in diploid and octoploid strawberry plants, we adopted a method developed by Iwatsubo and Naruhashi (1991) for diploid strawberries. Fig. 1A shows that many clear images of diploid plants were obtained using that method. However, for octoploid plants, there arose various difficulties, mostly because the root tips of octoploid plants were thicker and harder than those of the diploid plants: (1) overlapped cells by insufficient softening treatment, (2) overlapped chromosomes and unclear imaging attributable to contamination with cytoplasm, and (3) unclear images of pro-metaphase chromosomes.
3. Result and discussion3.1. Lacto-propionic orcein stainingFirst, for the experiments of chromosome observation at the metaphase stage in diploid and octoploid strawberry plants, we adopted a method developed by Iwatsubo and Naruhashi (1991) for diploid strawberries. Fig. 1A shows that many clear images of diploid plants were obtained using that method. However, for octoploid plants, there arose various difficulties, mostly because the root tips of octoploid plants were thicker and harder than those of the diploid plants: (1) overlapped cells by insufficient softening treatment, (2) overlapped chromosomes and unclear imaging attributable to contamination with cytoplasm, and (3) unclear images of pro-metaphase chromosomes.
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