The p25 coat protein (CP) gene of Citrus tristeza virus (CTV) was incorporated to Mexican lime plants and
forty-two transgenic lines were produced, 25 containing the p25 CP gene of the severe CTV strain T-305 and 17
with that of the mild strain T-317. When plants propagated from each transgenic line were graft-inoculated with
CTV T-305 or aphidinoculated with T-300, two types of response to viral challenge were observed: some lines
developed CTV symptoms similar to those of non-transgenic controls, whereas others exhibited protection against
the virus. This protection consisted of a proportion of plants, ranging from 10 to 33%, that were resistant to CTV,
and the rest of them that showed a significant delay in virus accumulation and symptom onset. Protection was
efficient against non-homologous CTV strains and was generally accompanied by high accumulation of p25 CP
in the protected lines, which suggest a CP-mediated protection mechanism in most cases. This is the first report
demonstrating pathogen-derived resistance in transgenic plants against a Closterovirus member in its natural host.
The p25 coat protein (CP) gene of Citrus tristeza virus (CTV) was incorporated to Mexican lime plants andforty-two transgenic lines were produced, 25 containing the p25 CP gene of the severe CTV strain T-305 and 17with that of the mild strain T-317. When plants propagated from each transgenic line were graft-inoculated withCTV T-305 or aphidinoculated with T-300, two types of response to viral challenge were observed: some linesdeveloped CTV symptoms similar to those of non-transgenic controls, whereas others exhibited protection againstthe virus. This protection consisted of a proportion of plants, ranging from 10 to 33%, that were resistant to CTV,and the rest of them that showed a significant delay in virus accumulation and symptom onset. Protection wasefficient against non-homologous CTV strains and was generally accompanied by high accumulation of p25 CPin the protected lines, which suggest a CP-mediated protection mechanism in most cases. This is the first reportdemonstrating pathogen-derived resistance in transgenic plants against a Closterovirus member in its natural host.
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