Each reaction included 12.5 μl SYBR Green Supermix, 1 μl of each Primer, 1 μl of DNA samples and 9.5 μl molecular H2O. The reaction conditions for amplification of DNA were initial denaturation at 94◦C for 5 min,
Each reaction included 12.5 μl SYBR Green Supermix, 1 μl of each Primer, 1 μl of DNA samples and 9.5 μlmolecular H2O. The reaction conditions for amplification of DNA were initial denaturation at 94◦C for 5 min,