2.2.2. Minerals
One
gram
of
PDBF
was
digested
in
5 mL
of
concentrated
nitric
acid
and
swelled
to
50 mL
in
a
volumetric
flask
. After
filtration,
calcium
(Ca),
magnesium (Mg),
iron (Fe),
copper
(Cu),
zinc (Zn)
and
sodium
(Na)
were
determined
by
Inductively
Coupled
Plasma-
Atomic
Emission
Spectrometry,
using
Spectroflame
FVM03
(Spectro
Analytical
Instruments),
equipped
with
a
vacuum
polychromator
and
in
air
with
network
holographic
monochromator
with
2400
grooves/mm,
using
a
Meinhard
nebulizer.
Standard
curves were
expressed
in
mg/100 g.