Figure 3 suggested that the level of trehalose increased with the changes of the salt concentration within a certain range. The original strains S
and T could accumulate most trehalose in corn juice in 9% salt, while the mutants was in 12% salt. And if out
of the certain range, it would decrease which might be due to that trehalose was used as a carbon source, or
the metabolism of yeast changed and the glucose was used to synthesize other compatibility substances.
Heat shock could induce the synthesis of trehalose-6-phosphate synthase and trehalose-6-phosphate
phosphatase. So heat shock was on the basic of salt stimulation. Figure 4(a) showed that the trehalose level of
S in 9% salt changed significantly with different temperature and different stimulation time, and the other
original yeast T displayed the same trend (Fig 4(b)) that the trehalose level reached the peak at 40
temperature for 30 mins. To research the relevance of the salt tolerance and trehalose level, the original
strains cultivated in 9% salt and the mutants in 12% were stimulated for 30 mins by different temperature.
And the results were shown in Figure 5, it could make a conclusion that although salt and heat shock were
able to improve the accumulation of trehalose in yeasts, the mutants produced less than the original ones. This
proved that the gene of trehalose in the mutants might be not changed and the salt-tolerance of salt-resist yeast
had less relationship with the level of trehalose. In the mutant strains, the substrate might be synthesis other
substances such as glycerol or the like.
Figure 3 suggested that the level of trehalose increased with the changes of the salt concentration within a certain range. The original strains S
and T could accumulate most trehalose in corn juice in 9% salt, while the mutants was in 12% salt. And if out
of the certain range, it would decrease which might be due to that trehalose was used as a carbon source, or
the metabolism of yeast changed and the glucose was used to synthesize other compatibility substances.
Heat shock could induce the synthesis of trehalose-6-phosphate synthase and trehalose-6-phosphate
phosphatase. So heat shock was on the basic of salt stimulation. Figure 4(a) showed that the trehalose level of
S in 9% salt changed significantly with different temperature and different stimulation time, and the other
original yeast T displayed the same trend (Fig 4(b)) that the trehalose level reached the peak at 40
temperature for 30 mins. To research the relevance of the salt tolerance and trehalose level, the original
strains cultivated in 9% salt and the mutants in 12% were stimulated for 30 mins by different temperature.
And the results were shown in Figure 5, it could make a conclusion that although salt and heat shock were
able to improve the accumulation of trehalose in yeasts, the mutants produced less than the original ones. This
proved that the gene of trehalose in the mutants might be not changed and the salt-tolerance of salt-resist yeast
had less relationship with the level of trehalose. In the mutant strains, the substrate might be synthesis other
substances such as glycerol or the like.
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