Flasks of nutrient medium are first sterilized under high heat and pressure to kill any fungal or bacterial contaminants. The medium in the containers is made of sugars, inorganic salts, plant hormones and a gelling agent (like agar).
The plant material used to start a culture is called an explant and must be disinfected before being placed on a sterile medium.
To maintain the sterility of the plant cultures, the containers are opened only in a laminar flow hood, which contains a high-efficiency particle absorption (HEPA) filter. This creates an environment free of airborne organisms that could contaminate the tissue culture.
The container of nutrient medium is first sterilized under high heat and pressure to kill any fungal or bacterial contaminants.
After the culture has been started, it is stored under lights and carefully monitored for contamination.
After a period of days or up to several months, the explants will show signs of growth. Leaves, roots and other organs will begin to develop.
In time, under the proper conditions, the cultures will grow and multiply. They can be divided or subcultured into new flasks as the nutrients in the medium eventually run out. This process can go on indefinitely, producing many plants over time.
Plants grown in the laboratory can be transferred to soil conditions carefully by selecting the correct soil mix and gradually lowering the humidity level.
Flasks of nutrient medium are first sterilized under high heat and pressure to kill any fungal or bacterial contaminants. The medium in the containers is made of sugars, inorganic salts, plant hormones and a gelling agent (like agar).The plant material used to start a culture is called an explant and must be disinfected before being placed on a sterile medium.To maintain the sterility of the plant cultures, the containers are opened only in a laminar flow hood, which contains a high-efficiency particle absorption (HEPA) filter. This creates an environment free of airborne organisms that could contaminate the tissue culture. The container of nutrient medium is first sterilized under high heat and pressure to kill any fungal or bacterial contaminants. After the culture has been started, it is stored under lights and carefully monitored for contamination. After a period of days or up to several months, the explants will show signs of growth. Leaves, roots and other organs will begin to develop. In time, under the proper conditions, the cultures will grow and multiply. They can be divided or subcultured into new flasks as the nutrients in the medium eventually run out. This process can go on indefinitely, producing many plants over time. Plants grown in the laboratory can be transferred to soil conditions carefully by selecting the correct soil mix and gradually lowering the humidity level.
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