and 2 U Taq polymerase (MBI Fermentas) to a total
volume of 25 μl in a 96-well PCR plate. The PCR reactions were subjected
to an initial denaturation for 5 min at 95 °C, followed by 35 cycles of
amplification in an AB 2720 thermal cycler: denaturing for 30 s at
95 °C, annealing for 1 min at 65 °C and extension for 30 s at 72 °C