the initial estimates given in the analysis are the spectra of AuNPs and AuNPs-HSA conjugate resolved above.the final lack of fit,variance explained and similarity coefficient obtained are all below 0.30%,above 99.99% and above 0.9999,respectively,suggesting the validity of all resolutions. combined with known information about stoichiometric numbers of all species in the reaction and the initial molar concentration of AuNPs,the konetic profiles of all species in terms of relation concentrations resolved by MCR-ALS can be converted into the desired kintic profiles in terms of absolute molar concentration.As can be noticed from the three figures,during the binding process of HSA to the AuNPs surface, the concentrations of AuNPs and HSA decrease exponentially while those of AuNPs-HSA conjugate increase exponentially.in addition,it is clearly seen that the higher the concentrations of HSA,the faster the binding reaction.