The milled jackfruit seeds were
stored in plastic bag at 4 °C. Then, 50% (w/v) jackfruit seeds starch in distilled water was
supplemented with additional nutrients to give a medium composition of 0.5 gL-1 MgSO4.7H20,
0.5 gL-1 KH2PO4, 12.5 gL-1 (NH4)2SO4. A medium was sterilized at 121 °C for 15 min. The fungi
hydrolysis was carried out in 250 mL Erlenmeyer flasks containing 20 g jackfruit seed starch and
40 ml distilled water with additional nutrients. All fungi were cultivated on PDA slant and
incubated at 30 °C for 7 days and spore suspension of these fungi were used for starter culture.
Then, 10% aliquot of the starter culture was added separately to the medium. The culture was
incubated at 30 °C for 72 h and 168 h. The amount of reducing sugar produced was determined
using the Somogyi-Nelson method according to Nelson [11]. At least three samples were used in
all analytical determinations and data were presented as the mean of three replications.