Thereafter, embryos were transferred into a 10-L droplet of DPBS containing 90% (v/v) glycerol (Nacalai) and 1.9 M Hoechst 33 342 (Sigma- Aldrich) on a glass slide
Thereafter, embryos were transferred into a10-L droplet of DPBS containing 90% (v/v) glycerol(Nacalai) and 1.9 M Hoechst 33 342 (Sigma-Aldrich) on a glass slide