Things to do before starting
■ Equilibrate Buffer ATE or distilled water for elution to room temperature (15–25°C).
■ Set a thermomixer or heated orbital incubator to 56°C for use in step 3 and
(optional) step 16, and a second thermomixer or heated orbital incubator to 70°C
for use in step 6. If thermomixers or heated orbital incubators are not available,
heating blocks or water baths can be used instead.
■ If Buffer AL or Buffer ATL contains precipitates, dissolve by heating to 70°C with
gentle agitation.
■ Ensure that Buffers AW1 and AW2 have been prepared according to the
instructions on page 11