Quantitative Estimation of Diacetyl Production
The amount of diacetyl produced during the fermentation of the samples was also determined by titration: 25 ml of the fermented sample and 7.5 ml hydroxylamine solution were titrated against 0.1 M HCl according to a standard procedure (AOAC 1990). The equivalent factor of HCl to diacetyl was taken as 21.5 mg.