Freeze-dried of Lactobacillus acidophillus FBRL-B07
was activated in MRS (Man Rogosa Sharp) broth at 37°C for
48 h [7]. Single colonies were selected and grown on MRS
agar at 37°C for 48 h. Sterilize peptone water was then used
to dilute and obtain optical density (OD) of 1.8-1.9 at 600 nm.