Thermal processing of Low Acid Canned Foods (LACF), which are safe and shelf-stable at ambient temperature for
several years, results in heat inactivation of all vegetative microorganisms and the partial or total inactivation of
spores. Good Manufacturing Hygienic Practices include stability tests for managing the pathogen risk related to surviving
mesophilic bacterial spores. LACF are also often submitted to additional incubation conditions, typically 55 °C
for 7 days, to monitor spoilage by thermophiles. In this study we identified the bacterial species responsible for
non-stability after prolonged at 55 °C of incubation of LACF from 455 samples collected from 122 French canneries
over 10 years.