Effect of pretreatment on compositional change of Miscanthus Raw Miscanthus was mainly composed of 40.5 wt% cellulose, 23.8 wt% hemicellulose and 24.1 wt% lignin (Fig. 4-a). Alkaline pretreatment of Miscanthus was conducted with 1.5 M NaOH at 150 C for 30 min. In result, 42.6% of pretreated solids were recovered. Following the pretreatment, cellulose content of pretreated Miscanthus drastically increased from 40.5 wt% to 77.9 wt%. In contrast, the lignin content was significantly reduced from 24.1 wt% to 8.0 wt% by the pretreatment, Considering that the degree of delignification of pretreated Miscanthus, alkaline pretreatment in this study may be reasonable approach to evaluate the fermentability of S.ceevisiae mbc 2 (Fig. 4-a). In addition, the evident increase in cellulose content was notable because S.cerevisiae mbc 2 only utilized glucose foe SSF using Miscanthus.