In vitro antioxidant activity of Centella asiatica (Linn.) and the impact of extraction solvent polarity on the
antioxidant potential were investigated in the present study. 100% ethanol, 50% ethanol and water were chosen as
extraction solvent due to arithmetic progression of their polarity. Total polyphenol, flavonoid, β-carotene, tannin and
vitamin C content of these three extracts were determined while their antioxidant potentials were assayed by total
reducing power assay and 2, 2-diphenyl-1-picrylhydrazyl (DPPH)-scavenging activity. 50% ethanol extract of C.
asiatica contained significantly higher amount of polyphenol, flavonoid while moderate amount of carotene and
tannin but the lowest amount of vitamin C compared to 100% ethanol and water extract. All the phytochemicals
showed solvent polarity specific extraction pattern. Total reducing power and DPPH-radical scavenging activity of
50% ethanol extract also were significantly higher when compared to those of the 100% ethanol and water extracts.
Significant variations of antioxidant potentials of C. asiatica due to differences in the extraction solvent polarity
were demonstrated in this study