After
addition of the solutions, OPF were inoculated with WRF cultures and incubated at 30 ◦C for 3 or 9 wks. Each treatment was
inoculated in duplicate for each colonization period. Besides treatments with the 10 WRF, an autoclaved control without
fungal inoculum was included which was incubated in duplicate for 9 wks. Controls which were incubated for 3 wks were
lost due to a laboratory error.