The callus induction and plantlet regeneration for
C. nutans is not easy to achieved (Ying, 2013;
Gunasekaran, 2014). The extracts from the callus and
suspension cells used for drugs are controversial since
it may contain residual composition of plant growth
regulators.
Compared to other medicinal plants, tissue
culture technique of C. nutans achieved more easily
than that of Tripterygium wilfordii and Dendrobium
officinale (Chen, 2009; Chen et al, 2014).