Ethanol and total sugars were determined using a high- performance liquid chromatography (LaChrom Elite, Hitachi High-Technologies Corp., Tokyo) equipped with a packed column (Shodex KS-801, Showa Denko Co., Tokyo) and an RI monitor. The analytical condition for individual sugars was as follows: column, Asahipack NH2P-50 4E (4.6 mm x 250 mm, Showa Denko Co., Ltd., Tokyo); column temperature, 50 o C; mobile phase, acetonitrile:water ¼ 75:25; flow rate,
1.0 mL min-1.
Whole and viable numbers of yeast cells were counted using a hemocytometer with and without staining by the alkaline-methylene blue reagent [9].
Data are shown as the average values from two indepen- dent experiments unless otherwise stated.
Ethanol and total sugars were determined using a high- performance liquid chromatography (LaChrom Elite, Hitachi High-Technologies Corp., Tokyo) equipped with a packed column (Shodex KS-801, Showa Denko Co., Tokyo) and an RI monitor. The analytical condition for individual sugars was as follows: column, Asahipack NH2P-50 4E (4.6 mm x 250 mm, Showa Denko Co., Ltd., Tokyo); column temperature, 50 o C; mobile phase, acetonitrile:water ¼ 75:25; flow rate,1.0 mL min-1.Whole and viable numbers of yeast cells were counted using a hemocytometer with and without staining by the alkaline-methylene blue reagent [9].Data are shown as the average values from two indepen- dent experiments unless otherwise stated.
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