Due to phosphate and carboxylic moieties in LPS,the gram-negative bacteria cell surface is negatively charged. Divalent cation such as Mg cross -link LPS,thus stabilizing OM structure. The OM becomes more permeable to hydrophobic compound when the cation are removed using chelating agents such as EDTA. In addition to LPS and phospholipids,the OM contains various proteins which have various functions including lipoprotein
Active transport of nutrients takes place across the cytoplasmic membrane. Nutrients therefore need to cross the OM,and for this the OM has porins and receptors. Porins are hydrophilic channels formed by three peptides spanning the OM,allowing the diffusion of solutes of molecular weight less than 600Da. E.coli has three porins,omp,ompf and phoe-ompc and ompf being non-specific. Ompf is slightly bigger than ompc,the synthesis of which is stimulated under high osmotic pressure when the synthetic of the former is repressed
Due to phosphate and carboxylic moieties in LPS,the gram-negative bacteria cell surface is negatively charged. Divalent cation such as Mg cross -link LPS,thus stabilizing OM structure. The OM becomes more permeable to hydrophobic compound when the cation are removed using chelating agents such as EDTA. In addition to LPS and phospholipids,the OM contains various proteins which have various functions including lipoprotein Active transport of nutrients takes place across the cytoplasmic membrane. Nutrients therefore need to cross the OM,and for this the OM has porins and receptors. Porins are hydrophilic channels formed by three peptides spanning the OM,allowing the diffusion of solutes of molecular weight less than 600Da. E.coli has three porins,omp,ompf and phoe-ompc and ompf being non-specific. Ompf is slightly bigger than ompc,the synthesis of which is stimulated under high osmotic pressure when the synthetic of the former is repressed
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