At present, availability of validated protocols to segregate psychrophiles and psychrotolerant bacteria from mesophiles or thermophiles is strictly limited. Conclusive characterization as psychrophiles, psychrotolerants or mesophiles is impractical as hitherto available data is limited to specific taxonomic groups of bacteria or their proteins. Global cellular responses expressed specifically by psychrotolerant or psychrophilic bacteria to cold stress, have not yet been reported [37]. Attempts on comparing the proteins from psychrophilic, and mesophilic bacteria have produced ambiguous results, and crystallographic structures of psychrophilic proteins indicate a high percentage similarity in their conformation to their mesophilic counterparts [38]. Genetic screening of bacteria using PCR for specific marker sequences which act as signature sequences for psychrotolerants have also been carried out in the past [39,40]. However, the primers used in these PCR assays were genus or species specific, and thus cannot broadly delineate the physiological trait (psychrophile or psychrotolerants) in bacteria belonging to other genera.