In order to study the carotenoid contents within calluses of YF and WF varieties, carotenoid content of both calluses was HPLC analyzed (Fig. 6). HPLC profile of the YF variety callus showed two main peaks (Fig. 6a). Lutein was eluted at 23.4 min, and zeaxanthin at 26.5 min. Pigment profiles corresponded to absorbance spectra (Fig. 6c). HPLC profile of the WF callus showed a main peak at 23.6 min corresponding to lutein and some minor peaks at 26.1, 37.6 and 47.2 min, which match with retention times and absorption spectra corresponding to zeaxanthin,β-cryptoxanthin and β-carotene, respectively (Fig. 6b). Lutein was the main pigment found in both extract varieties.