5. Conclusion
In conclusion, this is the first evidence for the effect of
Agarwood essential oil on apoptosis and in vitro metastasis
Figure 7 The photomicrographs depict the images of HCT 116 cells with Rhodamin 123 stain, taken under an inverted phase-contrast
microscope at 200· magnification using a digital camera at 6 and 12 h after treatment with essential oils. (A) Cells treated with vehicle
(0.1% DMSO). (B) Cells treated with essential oils 10 lg/ml after 6 h. (C) Cells treated with essential oils 10 lg/ml after 12 h. (D) Cells
treated with standard reference, 5-flourouracil. (E) Graphical representation of percentage of apoptotic indices. The apoptotic index was
expressed as a percentage of the ratio of number of unstained cells to the total number of cell in 10 different microscopic fields. Values are
presented as mean ± SD (n = 10), * represents p < 0.05 and ** represents p < 0.01.
In vitro antimetastatic activity of Agarwood (Aquilaria crassna) essential oils 149
of pancreatic cancer cells.