The dengue fed- (DF) and blood-fed (BF) mosquitoes were used in this study. The Individual mosquito in each groups, was held in a small transparent plastic cup covered with nylon netting. The mosquito's feeding behavior was observed at an ambient room temperature. The mosquitoes were deprived of 10% sucrose solution overnight prior to assay. Mosquitoes were allowed to rest for at least 10 min before normal blood-feeding via an artificial membrane feeder apparatus. . The significance of difference was tested by student's t-test, one-way ANOvA, Chi- square depending on the data. P value