The enzymatic treatment with TG of the ice cream samples with 4, 6 and 8 g/100 g fat led to an increase in the overrun, partial coalescence of fat globules, melting resistance and hardness compared with the samples without enzyme treatment. Regarding the rheological parameters, protein polymerization induced by TG favored the pseudoplastic properties of the ice cream and gave higher values for the apparent viscosity, consistency index, hysteresis and initial tension required to initiate the structural break of the samples. The addition of transglutaminase led to the ice cream samples with 4 g/100 g and 8 g/100 g fat having similar characteristics, indicating that the enzyme can be used as a partial replacement for fat in ice cream, without affecting the functional and rheological properties of these products