Moreover the calibration curve is prepared type external standard and matrix math standard before take two type of t-test. As a result, two type of calibration curve is same (tstat < tcritical) So, calibration curve can prepare with external standard.The reagents was tested for real urine samples (non-spiked and spiked). The spectra quenching give a result of % recovery 90-102%, that is show reliability.The evaluated limits of detection and limits of quantitative is 0.08 and 0.3 mM glucose standard respectively.
The spectra of the steady state fluorescence experiments at different concentraions of hydrogen peroxide at constant curcumin concentration are shown in fig. As indicated by the arrow in this figure, the fluorescence intensity of curcumin is seen to decrese with increasing hydrogen peroxide (quencher)concentration