3.2.2. Effects of sample and reagent volumes on sensitivity
The effects of sample and reagent (acetate buffer pH 6, ECR and
DTAB) volume (Fig. 5(a)) on sensitivity of the system were studied.
The acetate buffer, ECR and DTAB volumes of 40, 50 and 40 mL were
selected based on its high peak height as optimum conditions.
After optimization of DTAB, ECR reagent and buffer volume, the
sample volume could be increased from 150 mL to 170 mL, while
the total volume was fixed at 300 mL.