Treated tomatoes were placed in individual sterile stomacher bags containing 20 ml of
universal preenrichment broth. Tomatoes were
manually shaken for 30 s, hand-rubbed for 30 s at the inoculation spot, and shaken for another 30
s. Serial dilutions were made using 9 ml of universal preenrichment broth and dilutions were
spiral plated onto TSAR. All plates were
incubated at 35°C for 24 h before enumerating surviving Salmonella. Negative control tomatoes
were plated on both TSA and TSAR plates to check for background microflora.