The effects of cryoprotectant (DMSO) on sperm were studied. Samples from the collected
sperm were prepared and mixed with HBSS extender at a ratio of 1:6 v/v (sperm: extender). Different
amounts of DMSO (5, 8 and 10 %) were then added and the mixture kept at 15oC. The motility grade
was then examined after 12, 24, 48, 72, 96 and 120 hours.