The effects of persimmon tannin and its characteristic units
on cell viability of L02 were shown in Fig. 2. After 48 h of treatment,
persimmon tannin showed obvious cytotoxicity on L02
cell viability at concentration of 40 g/ml, but at concentration
lower than 20 g/ml, persimmon tannin showed no
cytotoxicity. Similar as persimmon tannin, A-type EC dimer
showed no significant effect on cell viability of L02 upon 24 h
with concentrations less than 20 g/ml. While B-type EC dimer,
A-type ECG dimer and A-type EGCG dimer showed little effect
on cell viability up to 60 g/ml. Therefore, the concentrations
we used for persimmon tannin and A-type EC dimer were below
20 g/ml and for B-type EC dimer, A-type ECG dimer and A-type
EGCG dimer were less than 60 g/ml