2.9.2. Protein nutritional profiles, protein subfractions and
estimated energy studies
All chemical composition, protein subfractions partitioned by
CNCPS and estimated energy values data were analyzed by
using the PROC MIXED procedure of SAS 9.3 [30]. The model
used for analysis was:
Yij ¼ m þ Fi þ eij;
where Yij was an observation of the dependent variable ij; m
was the population mean for the variable; Fi was the feed
source (i ¼ 17), as a fixed effect, and eij was the random error
associated with the observation ij. The comparison of corn
based DDGS with barley based DDGS was carried out using
Contrast statement in SAS.
2.9.3. Protein rumen degradation and intestinal digestible
analysis
Statistical analyses were performed using the MIXED procedure
of SAS 9.3 [30]. The model for the analysis was:
Yijk ¼ m þ Fi þ Rj þ eijk,
where Yijk was an observation of the dependent variable ijk;
m was the population mean for the variable; Fi was the feed
source (i ¼ 17), as a fixed effect; Rj was the in situ experimental
cow as a random effect, and eijk was the random error
associated with the observation ijk. The comparison between
corn DDGS and barley DDGS was carried out using Contrast
statement in SAS.
2.9.4. Correlation analysis
The relationship of protein molecular structure (in terms of
amide I and amide II intensity and their ratio, a-helix and bsheet
intensity and their ratio) to changes in chemical and
nutrients profiles (in terms of chemical composition, protein
fractions partitioned by CNCPS, in situ rumen degradation
and estimated intestinal protein digestion in the different
2.9.2. Protein nutritional profiles, protein subfractions and
estimated energy studies
All chemical composition, protein subfractions partitioned by
CNCPS and estimated energy values data were analyzed by
using the PROC MIXED procedure of SAS 9.3 [30]. The model
used for analysis was:
Yij ¼ m þ Fi þ eij;
where Yij was an observation of the dependent variable ij; m
was the population mean for the variable; Fi was the feed
source (i ¼ 17), as a fixed effect, and eij was the random error
associated with the observation ij. The comparison of corn
based DDGS with barley based DDGS was carried out using
Contrast statement in SAS.
2.9.3. Protein rumen degradation and intestinal digestible
analysis
Statistical analyses were performed using the MIXED procedure
of SAS 9.3 [30]. The model for the analysis was:
Yijk ¼ m þ Fi þ Rj þ eijk,
where Yijk was an observation of the dependent variable ijk;
m was the population mean for the variable; Fi was the feed
source (i ¼ 17), as a fixed effect; Rj was the in situ experimental
cow as a random effect, and eijk was the random error
associated with the observation ijk. The comparison between
corn DDGS and barley DDGS was carried out using Contrast
statement in SAS.
2.9.4. Correlation analysis
The relationship of protein molecular structure (in terms of
amide I and amide II intensity and their ratio, a-helix and bsheet
intensity and their ratio) to changes in chemical and
nutrients profiles (in terms of chemical composition, protein
fractions partitioned by CNCPS, in situ rumen degradation
and estimated intestinal protein digestion in the different
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