Soybean meal was used to isolate resistant starch and produce nanoparticles, which could be potential
coating materials for colonic nutrient and drug deliveries. The nanoparticles were in 40 ± 33.2 nm
ranges. These nanoparticles were stable under simulated human physiological conditions. The degrees
of dissolution in both stomach and intestinal conditions were less than 30%. Furthermore, the nanoparticles
were less susceptible to pancreatic enzymatic digestion (20%), which was also evidenced by the
co-existence of B-type crystalline pattern. In addition to the dissolution and digestion studies in the
upper gastrointestinal tract, the nanoparticles were subjected to in vitro fermentation by Bifidobacterium
brevis and Lactobacillus casei. Both species showed an increase in growth and activity, while producing
short chain fatty acids: acetate, propionate, and butyrates in varying amounts. Overall this study clearly
demonstrated a novel method that can be used for colon-specific delivery of bioactive compounds such
as drugs and nutrients.